Detection and pathogenicity of Vibrio parahaemolyticus strains in Penaeus vannamei

Background and purpose: The Vibrio spp. are indigenous bacteria in the marine environment usually constitute majority normal microflora of farmed wild penaeid shrimp; one most diverse vital shrimp pathogens that cause heavy mortalities aquaculture facilities worldwide. present study aimed to isolate characterize causative agent concerned with mass mortality Peneaus vannamei collected from farms Kakinada, Andhra Pradesh, India.Materials methods: To pathogenic hepatic pancreatic tissue moribund, P. performed conventional culture tests, physical biochemical molecular analysis. In addition virulence gene study, bacterial pathogenicity different salinity antibacterial activity stem extract Tinospora cordifolia against parahaemolyticus were tested.Results: phenotypic traits, 16s rRNA gene, phylogenetic tree analysis showed isolated pathogen was V. strain KKD 02. A on 0, 5, 10, 15, 20, 25 ppt 5 10 connected maximum resistance infection higher CFU/shrimp value. Two genes encoding thermostable direct hemolysin (tdh) hemolysin-related (trh) isolate. T. cordifolia, tested parahaemolyticus, revealed both ethanol extracts crude juice exhibited parahaemolyticus.Conclusion: for juvenile India, Further work is necessary purify active constituents examine absorption pattern ingredients these plants, which will allow scientific community recommend their utilization as an accessible alternative synthetic antibiotics.